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KMID : 0352819890050010041
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Kosin Medical Journal
1989 Volume.5 No. 1 p.41 ~ p.53
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Infuluence of Estrogen or Progesterone on Growth and Adherence of Ureaplasma urealyticum to FL cell
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Abstract
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1
Many ivestigators have sugested that hormonal status are closely related with colonization rates of Ureaplasma urealyticum(U. urealyticum) in fermale genital tract, but some investigators do not agree with this view.
The present study was undertaken to test the significance of estrogen and progesterone acting as either stimulant or inhibitor on growth and adherence of U. urealyticum to FL cell in minimum essential medium(MEM) and ureaplasma standard liquid medium 10-B media.
U. urealyticum T960 strain and human amniotic fibroblast(FL) cells were used in this study. U. urealyticum was cultured in a 10-B medium formulated by Shepard and Lunceford. FL cells were cultured in complete MEM supplemented by in dose of 10% fetal calf serum and suitable antibiotics.
Estrogen was supplemented with 0.06ng, 0.6ng, 6.0ng or 60.0ng per ml and progesterone in dose of 0.1ng, 1.0ng, 10.0ng or 100.0ng per ml in 10-B or MEM media or FL cell culture system.
U. urealyticum was inoculated into 10ml of new 10- or MEM media containing different concentrations or estrogen or progesterone, and incubated at 37℃ for 30 hours. At every 6 hour interval, the bacterial number in the culture fluid was calculated by color changing unit(CCU). The CCU of culture fluid was determined by making serial ten-fold dilutions up to 10^(12) in duplicates with 10-B medial. The highest dilution at which one of the twom media changed from yellow to red was considered to contain 50% color changing unit.
The 90ml of FL cell suspension(10^(6)) were divided into 9 portions, and were added different concentration of estrogen and progesterone in each portion, then a 0.1 ml of U.urealyticum cluture fluid was added in it.
After, each portion was divided itno 1 ml and placed in 24 well culture plate, then incubated at 37℃for 72 hours. Bacterial number in the culture supermatant and adhered to cell were calcuated same as mentioned above.
The results of this study are summarized as follows
1. Growth of U.urealyticum in 10-B media was not influenced by 0.06ng or 0.6ng per ml of estrogen by 0.1ng or 1.0ng per ml of progesterone. Howerver, it was inhibited by high concentrations of estrogen and by progesterone.
2. Growth of U.urealyticum in MEM media was not affected by 0.06mg per ml of estrogen, but it was inhibited by high concentrations of estrogen and progesterone.
3. Growth of U.urealyticum in supermatant of FL cell culture system was not influenced by 0.06ng per ml of estrogen, while its survival times were prolonged by high concentrations of estrogen. On the other hand, adhesion of U.urealyticum to FL cells persistancy of the orgenism was increased and prolonged by high concentrations of estrogen.
4. Growth of U.urealyticum in supermatant of FL cell culture system was not increased, but its survival times were prolonged by progesterone. One the other hand, adhered U. urealyticum to FL cells were increased and its survival times were prolonged by progesterone.
Overall, we can suggest that the growth of U. urealyticum was inhibited by high concentrations of estrogen and progester one in 10-B or MEM media.
On the other hand, in FL cell culture system, the adherence of U. urealyticum to FL cells were increased and its survival times were prolonged by administration of estrogen and progestrogene.
We can also suggest that hormonal status is closely related with colonization of U. urealyticum in female genital tract.
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KEYWORD
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